biomolbioandco

Science, discussed.

Synthetic biology made circular [preprint]

Clever use of group I permuted intron-exon sequences to obtain circular riboregulators that performs better than their linear counterparts.

circulaeregulators

For the whole story, read the preprint on @bioRxiv
Engineering a circular riboregulator in Escherichia coli
William Rostain, Shensi Shen, Teresa Cordero, Guillermo Rodrigo, Alfonso Jaramillo

ABSTRACT
Circular RNAs have recently been shown to be important gene expression regulators in mammalian cells. However, their role in prokaryotes remains elusive. Here, we engineered a synthetic riboregulator that self-splice to produce a circular molecule, exploiting group I permuted intron-exon (PIE) sequences. We demonstrated that the resulting circular riboregulator can activate gene expression, showing increased dynamic range compared to the linear form. We characterized the system with a fluorescent reporter and with an antibiotic resistance marker. Thanks to the increased regulatory activity by higher stability, isolation due to self-splicing, and modularity of PIE, we envisage engineered circular riboregulators in further synthetic biology applications.

Advertisements

About Pietro Gatti

Interested in discussing (good) Science Lover of coffee & good films. Ideas all & only my own.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s

Information

This entry was posted on 25/09/2014 by in preprintreview, Synthetic Biology.

License

Creative Commons License
This blog is licensed under a Creative Commons Attribution 3.0 License.

Archives

Enter your email address to follow this blog and receive notifications of new posts by email.

Join 1,560 other followers

All Categories

%d bloggers like this: