Today I’m briefly mentioning a recently published and very comprehensive Annual Review in Pharmacology and Toxicology:
Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy Andreas Plückthun http://dx.doi.org/10.1146/annurev-pharmtox-010611-134654 Annual Review of Pharmacology and Toxicology, Vol. 55: 489 -511
For full disclosure, I’ll start saying that my interest is also due to my involvement in the isolation and characterisation of novel DARPins using SNAP display (Houlihan 2014, and more to come). However, these alternative binding scaffolds have a number of different applications, and the review gives a pretty good idea of what has been done, and what can be achieved.
Personally, I would have liked to see more time spent describing (with original experimental data) the construction and characterisation of DARPin, possibly including something on ankyrin repeat proteins in general. The review reads very much as an advertisement for applications, which is a fair aim for this sort of reviews (particularly considering that the author has created, patented and has shares in the company that commercialises DARPins) but does not fully trigger my curiosity.